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Braz. j. microbiol ; 43(2): 602-605, Apr.-June 2012. ilus
Article in English | LILACS | ID: lil-644476

ABSTRACT

Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.


Subject(s)
Animals , Dogs , Diagnostic Techniques and Procedures , Immunogenetics , In Vitro Techniques , Leptospira interrogans serovar canicola , Leptospirosis , Polymerase Chain Reaction/methods , Dogs , Methods
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